Step One

Carefully fill the plastic clean urine Mixing Container with lukewarm water (tap or bottled) up to the highest graduation line found just below the bottle neck (see bottle illustration above). The water used should be near room temperature (approximately 65°F to 70°F). It should not be too hot or too cold. Using near room temperature water will make it easier to gage the amount of Heat Activator needed in step 3.

Note that it is very important NOT to open Synthetic Sample or Heat Activator vials prior to use. Opening vials prior to use will significantly decrease the products effectiveness and its overall shelf life, also voiding its guarantee.

Step Two

Synthetic Sample- Add the entire contents of the Synthetic Sample vial to the water in the Mixing Container, screw cap on bottle and gently shake container until the powder has completely dissolved. (Synthetic Sample can be premixed with water up to six hours prior to testing).

Step Three

Heat Activator- Note that the use of the Heat Activator is optional; its sole purpose is to heat the solution to near body temperature. Therefore if prior to submitting your sample, you notice that the solution temperature is already within the proper temperature range of 92°F to 98°F (indicated in green) then you may skip Heat Activator step and move on to step 6.

IMPORTANT: If or when concealing the Mixing Container, never place the Container’s temperature strip next to or against your skin as this may lead to a false temperature reading!

For easy pouring; verify that the Heat Activator content is in a "loose" or "free flowing" granular form. If the granules are in a solid partially solid form, simply tap the vial against a hard surface, this break down any clumps and bring the Heat Activator back to it’s original granular form.

The principle behind the Heat Activator is: The lower the temperature of the solution = the more Heat Activator will be needed. However be very cautious as to the amount of heat activator used, you must avoid overheating the solution.

Guidelines to the amount of heat activator needed: If you do not see any temperature indication on the strip, this means your sample temperature is below 88°F. In this case we recommend that you carefully add 1/3 of the Heat Activator into the Mixing Container.

If the temperature on the strip indicates a solution temperature of 88°F to 92°F then we recommend that you only add 1/4 of the Heat Activator into the Mixing Container.

Caution: Adding an excessive amount of Heat Activator may overheat the solution; very rarely one will need to use more than 1/2 of the Heat Activator without overheating the solution! If the temperature of the solution rises above 98°F, let it cool down by laying the Mixing Container on the coldest surface available with temperature strip facing up. Wait until the temperature drops back down within the recommended range of 92°F to 98°F (indicated in green).

Step Four

After pouring the estimated amount of heat activator, replace and gently shake the contents to completely dissolve the Heat Activator, (usually takes 20 to 30 seconds).

After the Heat Activator granules have completely dissolved, read the temperature strip to ensure that the Solution is within the required temperature range of 92°F to 98°F (indicated in green).

Step Five

If necessary, carefully add a little more heat activator and repeat step 4.

Step Six

Once the Solution is in the acceptable temperature range, remove the cap of the Mixing Container and pour the required amount of Solution into the supplied specimen cup.